Yu etal_ISCIENCE-D-23-06561: A Non-Viral DNA Delivery System Consisting of Multifunctional Chimaeric Peptide Fused with Zinc Finger Protein

Published: 19 February 2024| Version 1 | DOI: 10.17632/jvgnzkwc7h.1
Siyuan Yu


Non-viral gene delivery systems have received sustained attention as a promising alternative to viral vectors for disease treatment and prevention in recent years. Numerous methods have been developed to enhance gene uptake and delivery in the cytoplasm, however, due to technical difficulties and delivery efficiency, these systems still face challenges in a range of biological applications, especially in vivo. To alleviate this challenge, we devised a novel system for gene delivery based on a recombinant protein eTAT-ZF9-NLS, which consisted of a multifunctional chimaeric peptide and a zinc finger protein with sequence-specific DNA-binding activity. High transfection efficiency was observed in several mammalian cells after intracellular delivery of plasmid containing ZF9-binding sites mediated by eTAT-ZF9-NLS. Our new approach provides a novel transfection strategy and the transfection efficiency was confirmed both in vitro and in vivo, making it a preferential transfection reagent for possible gene therapy. The excel files are arranged figure wise and contain the raw data used to generate figures shown in the manuscript "ISCIENCE-D-23-06561".


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Please see the Key resource table for reagent list and Method details in the manuscript ISCIENCE-D-23-06561. The Excel file details the data and statistical analysis involved in the manuscript.


Xiamen University School of Public Health


Gene Delivery, Transfection (Cell Biology), Zinc Finger, Cell-Penetrating Peptide


National Natural Science Foundation of China


National Natural Science Foundation of China


Fundamental Research Funds for the Central Universities