The immediate impact of exoribonucleolysis on nuclear RNA processing, turnover and transcriptional control revealed by rapid depletion of DIS3, EXOSC10 or XRN2 from human cells

Description

This data represents uncropped western and northern blots showing rapid depletion of EXOSC10 or DIS3 from human cells (westerns) and the effect of EXOSC10 loss on 5.8S rRNA processing (Northern).

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All western blots were blocked for 1hr in 5% milk in PBS tween except for the AID blot which is blocked overnight in 10% milk in PBS tween. All primary antibodies were used at 1:1000 and secondary antibodies used at 1:2500. Antibody probing was for 1hr at room temperature in 2% milk in PBS tween. PBS tween was used to wash blots 3x in between primary and secondary antibodies and 3x before exposure. Blots were imaged using a biorad gel doc system. The Northern blot protocol is published in the accompanying manuscript.

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