LentiCRISPR-Cas9-2A-mCherryU6ZF vector

Published: 29 November 2018| Version 1 | DOI: 10.17632/k25kdh7z25.1
sebastian escobar


The plasmid (15, 126 pb) was constructed encoding the spCas9 protein driven by the EFS (mammalian) promoter in a bicistronic cassette that also expressed mCherry. For the expression of the sgRNA, a cassette containing the zebrafish U6 RNA III polymerase (U6ZF) promoter was used. The new plasmid was able to display the expression for Cas9, mCherry and sgRNA in CHSE/F fish cell.