Phylogenetic assessment of the Metamasius hemipterus species complex (Coleoptera, Curculionidae, Dryophthorinae).

Published: 17 May 2022| Version 2 | DOI: 10.17632/k37bdb7dhj.2
Luciano Palmieri


We investigated the phylogenetics of the Metamasius hemipterus species complex, which comprises three weevil subspecies of a widely distributed agricultural pest. Several aspects of this species are uncharacteristic for weevils, such as its broad distribution and its compatibility with a wide array of host plants. Furthermore, the three subspecies of M. hemipterus are distinguishable only by color patterns, which may not constitute reliable indicators of species identity. We tested the validity of this species complex and its three-constituent subspecies, using a RAD-seq genome-skimming approach. Here, we show that Metamasius hemipterus carbonarius is a melanized form of M. h. sericeus; and that these two putative subspecies are genetically distinct from M. h. hemipterus. We revise the species limits of this complex, dividing it into two species (M. sericeus and M. hemipterus) and provide an updated taxonomic key.


Steps to reproduce

Ipyrad parameters input file (.txt) and outputs to serve as input on phylogenetic analysis (supermatrix = .phy; SNPs = .snps). Consensus output trees of IQTREE (.contree files). The raw Illumina reads are deposited at NCBI Sequence Read Archive (SRA) under the accession PRJNA819383.


University of Wisconsin Madison