Data for: MDA5 and LGP2 acts as a key regulator though activating NF-κB and IRF3 in RLRs signaling of Mandarinfish

Published: 29 Dec 2018 | Version 1 | DOI: 10.17632/k3ksdm8c6m.1
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Description of this data

Characterization of the effects of MDA5 and LGP2 on induction of IFN-β, NF-𝜅B and IRF3. MDA5 293T cells and LGP2 293T cells were seeded in 6 well plates and treated with 10ng/ml tetracycline, respectively, after seventy-two hours culturing, the cells were stimulated with poly I:C (1mg/mL) or LPS (1mg/mL). Ten hours later, the cells were treated with RIPA to carry out the western blot analysis. Gray value detection of western blot were repeated at least three times, and rubber map of western blot was used.

Experiment data files

This data is associated with the following publication:

MDA5 and LGP2 acts as a key regulator though activating NF-κB and IRF3 in RLRs signaling of mandarinfish

Published in: Fish and Shellfish Immunology

Latest version

  • Version 1

    2018-12-29

    Published: 2018-12-29

    DOI: 10.17632/k3ksdm8c6m.1

    Cite this dataset

    Xu, qi (2018), “Data for: MDA5 and LGP2 acts as a key regulator though activating NF-κB and IRF3 in RLRs signaling of Mandarinfish”, Mendeley Data, v1 http://dx.doi.org/10.17632/k3ksdm8c6m.1

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Western Blot

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