Data for: Toxic effects of 1-(N-methyl-N-nitrosamino)-1-(3-pyridinyl)-4-butanal on the maturation and subsequent development of murine oocyte

Published: 15 June 2019| Version 1 | DOI: 10.17632/k7fvyb3tjn.1
Yun-Peng Hou


NNA exposure reduced the polar body extrusion rate by leading 8-oxo-deoxyguanosine (8-OHdG) increasing, and disrupting the meiotic spindle morphology by inhibiting ERK1/2 activation during in vitro maturation. Additionally, NNA exposure resulted in cleavage and blastocyst rates reduction by altering DNA and histone methylations with reducing 5mC and H3K4me2 levels, and by inducing apoptosis caused by mitochondrial dysfunction and ROS accumulation, as shown by increased SOD mRNA level and decreased Bcl-x mRNA level. Taken together, our results demonstrate that NNA exposure reduces the maturation and developmental capability of murine oocytes by increasing the risk of DNA damage and abnormal spindle morphology, altering epigenetic modifications and inducing apoptosis, suggesting toxic effect of NNA on mammalian productive health.



Apoptosis, Mitochondrion, DNA Damage, Reactive Oxygen Species, Spindle, Oocyte Maturation, Epigenetic Modification