Data of RNA sequencing, hydroxyproline level and qPCR primer swquences from an experiment examining the influence of Poria cocos polysaccharides on NASH mice
The hydroxyproline level in all mice (Fig. S1). The qPCR primer sequences including GAPDH, CCL3, CCR1, TNF-alpha, TLR4 and CD11b (Table S1). The differentially expressed genes shared by MCS and HP groups compared to MCD group (Table S2). The KEGG pathways enriched by HP group compared to MCD group (Table S3).
Steps to reproduce
C57BL/6 mice received 4 weeks of methionine and choline deficient (MCD) diet to establish nonalcoholic steatohepatitis model or methionine-choline-sufficient (MCS) diet as controls. Mice were assigned to the MCS group, MCD group, low-dose Poria cocos polysaccharides (PCP) (LP) group and high-dose PCP (HP) group, and were administered corresponding medications via gavage. The hepatic hydroxyproline and RNA sequencing (RNA-Seq) was performed. The hydroxyproline level in all mice was detected by the assay kit (A030-2-1, Nanjing Jiancheng Bioengineering Institute, China). The thresholds for differential expression shared by MCS and HP groups compared to MCD group analysis were established at |log2 fold change|> 1 and P value < 0.05.The hypergeometric test was used to calculate the KEGG pathway enrichment analyses, which was conducted using the KEGG pathway database (http://www.kegg.jp/). QPCR was used to verify the RNA-Seq result. GAPDH was used as the housekeeping gene and all primers were purchased from Sangon Biotech (Shanghai) Co., Ltd. (Shanghai, China).