Presence of CD3+ and CD79a+ lymphocytes in the pituitary gland of dogs at post mortem examination

Published: 06-04-2020| Version 2 | DOI: 10.17632/k9b7rwyrfb.2
Contributors:
Marcus Blomqvist,
Cecilia Ley,
Karolina Karlsson,
Jeanette Hanson

Description

Dataset accompanying the paper "Presence of CD3+ and CD79a+ lymphocytes in the pituitary gland of dogs at post mortem examination". The dataset consists of a comma-separated values (CSV) file with data from 20 dogs recruited at routine necropsy to investigate presence and distribution of lymphocytes in pituitary glands in dogs with no suspicion of pituitary disease. The pituitary glands were collected at the Section of Pathology, Department of Biomedical Sciences and Veterinary Public Health, Swedish University of Agricultural Sciences, Uppsala, from November 2013 to February 2014, and April 2017 to September 2017. Formalin-fixed paraffin-embedded pituitary sections were stained with haematoxylin and eosin (H&E) or subjected to immunohistochemistry (IHC) using primary antibodies specific for the T-cell marker CD3 and B-cell marker CD79a. The number of CD3+ and CD79a+ cells per area unit (CPA, cells/mm²) was determined for different pituitary regions: pars tuberalis (PT), pars distalis (PD), pars intermedia (PI), infundibulum (In), and lobus nervosus (LN). The dataset contains information about: • Identification and signalement — fields: ID, Breed, Age (years of age at necropsy), Sex, Neutering status • Concurrent pathological findings — field: Necropsy diagnosis (multiple diagnoses are separated by semicolon) • Morphologic changes in the pituitary gland — fields: Pituitary lesion, Pituitary lesion group (No, Limited or Extensive lesion) • Number of CD3+ CPA (cells/mm²) in each pituitary region* — fields: PT CD3 CPA, PD CD3 CPA, PI CD3 CPA, In CD3 CPA, LN CD3 CPA • Number of CD79a+ CPA (cells/mm²) in each pituitary region* — fields: PT CD79a CPA, PD CD79a CPA, PI CD79a CPA, In CD79a CPA, LN CD79a CPA ____________ *PT, pars tuberalis; PD, pars distalis; PI, pars intermedia; In, infundibulum; LN, lobus nervosus

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