Dataset on exposure conditions to Fe2O3 and SiO2 colloidal suspension and airborne particulate matter (PM) suspensions: crude NIST1648a and with reduced content of organic matter, LAp120

Published: 3 September 2023| Version 1 | DOI: 10.17632/k9y6rz5ghh.1


Data for publication in Data in Brief. The dataset presents a comparative analysis of nebulization process of Fe2O3 and SiO2 nanoparticles or crude particular matters (PM), NIST1648a, and that with reduced content of organic matter, LAp120. Nebulization tests were carried out to determine concentrations of nanoparticle and PM suspensions, in order to create an atmosphere with a concentration of PM particles about 1000 μg/m3 of air in the exposure chambers. The experiments were performed on empty chambers in order to establish a proper exposure conditions for further experiments with laboratory animals. The Excel file spreadsheets show the raw data for each figure presented in the manuscript. Raw data in Fig. 1 shows the absorbance spectra of Fe2O3 and SiO2 nanoparticle suspensions and NIST1648a and LAp120 suspensions in the range of 300-700 nm compared to the absorbance of deionized water. The Fig. 2 presents the relationship between the optical density and the concentration of Fe2O3 and SiO2 nanoparticle suspensions as well as NIST1648a and LAp120 suspensions at a wavelength of 300 nm. The Fig. 3 shows the concentrations of Fe2O3 (A) and SiO2 (B) nanoparticle suspensions and NIST1648a (C) and LAp120 (D) suspensions, control, non-nebulized (Ctrl) and after passing through nebulizers (Neb1-4), determined at the wavelength of 300 nm and expressed as a percentage of the control suspensions. The Fig. 4 shows the PM10 concentration in empty chambers, without animals, during nebulization of nanoparticle suspensions Fe2O3 (0.4 mg/ml) and SiO2 (0.6 mg/ml) and suspensions of NIST1648a and LAp120 (both 1.2 mg/ml). The Fig. 5 presents the air temperature and relative humidity in empty chambers, without animals, while nebulizing LAp120 suspension (1.2 mg/ml). Nebulization of particle suspensions is one of the methods of introducing dust particles of air pollutants in experimental conditions into the atmosphere. The data are useful for everybody studying the influence of airborne PM or engineered nanoparticles on the laboratory animals through nebulization method.


Steps to reproduce

The nebulizers used in the present experiment produce aerosols with droplet sizes in the range of 4-6 μm (according to the manufacturer's instruction). The Fe2O3 and SiO2 nanoparticles are 4-8 and 10 nm in size, respectively. The average particle size of NIST1648a is 5.85 μm (according to the manufacturer's certificate) and cold plasma treatment had no effect on particle size or aggregation. Since some of NIST1648a and LAp120 will not be dispersed by nebulizers due to their size, it is necessary to determine the concentration of suspensions of these materials after passing through nebulizers. A turbidimetric method was used to estimate the concentration of suspensions passing through the nebulizers. As an assessment of the concentration of suspensions, the absorbance was measured with a Synergy MX microplate reader working with Gen5 software (both BioTek Instruments, Winooski, USA). Based on the obtained data, a wavelength of 300 nm was selected for further concentration studies. The choice of this wavelength was confirmed by measurement of absorbance on the concentration of suspensions. In the next step, the absorbance of control, non-nebulized suspensions, and suspensions after passing through nebulizers were measured, and from this, the extent to which particles pass through nebulizers and are dispersed in the exposure chamber was estimated. Nebulization tests were carried out in order to determine concentrations of particle suspensions and the parameters of the nebulizer's operation for recreate an atmosphere with a concentration of PM10 about 1000 μg/m3 of air in the exposure chambers without a substantial changes of other parameters of microclimate, i.e. temperature, humidity and CO2 concentration. Fifteen air exchanges per hour were applied in order to maintaining microclimate parameters that did not adversely affect welfare of mice during further studies. Experiments were carried out with DSI's Mass Dosing System (Data Sciences International, St Paul, USA) composed of exposure chamber equipped with Aerogen Aeroneb® Lab nebulizer (Aerogen Limited, Galway, Ireland) and outlet air filter, and controller for control of nebulizer and provides air exchange in the chamber. During nebulization, concentration of PM10 as well as temperature, the relative humidity and CO2 concentration were measured with Aerocet 831 handheld particle counter (Met One Instruments, Inc., Grants Pass, USA) and AZ77535 air parameters detector (AZ Instrument Corp., Taichung, Taiwan), respectively.


Polska Akademia Nauk Instytut Farmakologii w Krakowie


Toxicity, Air Pollutant, Environmental Pollution, Fine Particulate Matter


National Science Centre, Poland


Maj Institute of Pharmacology, Polish Academy of Sciences in Krakow, Poland

statutory funds