Files for "Localized Macrophage Stimulation with Small Molecule TLR Agonist via Fluidic Force Microscopy"

Description

Supporting data and code for "Localized Macrophage Stimulation with Small Molecule TLR Agonist via Fluidic Force Microscopy, " manuscript in preparation. Includes: example data and full code referenced in the paper, and diffusion modeling for the agonist R848 using AlexaFluor488. This protocol paper supports the manuscript "Evidence of Collective Influence in Innate Sensing using Fluidic Force Microscopy." The hypothesis of this work is that Fluidic Force Microscopy can be used for local immune cell stimulation in culture, on the order of single macrophages or small clusters of macrophages. The paper describes the full protocol for those experiments, quantification and analysis methods, and the method for determining the agonist concentration as it is dispensed and diffuses away from the dispensing point. For details on data interpretation, see the manuscript.

Steps to reproduce

For method details, see publication.

Institutions

• University of Chicago

Categories

Funders

• National Science Foundation

  Grant ID: DMR-2011854
• National Institute of Allergy and Infectious Diseases

  Grant ID: 75N93019C00041, 5U01AI124286-06
• Camille and Henry Dreyfus Foundation
• Defense Threat Reduction Agency

  Grant ID: HDTRA11810052
• Pew Charitable Trusts
• National Science Foundation

  Grant ID: OMA-2121044

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