Data for: Correlative FLIM-Confocal-Raman mapping applied to plant lignin composition and autofluorescence.

Published: 31-08-2019| Version 1 | DOI: 10.17632/kh837ypv2k.1
Contributors:
Raymond Wightman,
Marta Busse-Wicher,
Paul Dupree

Description

These are the raw data in their original filetypes as follows: Lignin_LASX_Leica.lif = The confocal images showing lignin autofluorescence in the WT and lignin mutant. The data were created in Leica LAS X software and additionally contain all the metadata describing the imaging parameters. The .lif file can additionally be read in Fiji/ImageJ. Lignin_FLIM.sptw.zip = ZIP archive of the SymPhoTime 64 workspace containing all the FLIM data of the same regions as the above confocal images taken in LAS X. Can be read by SymPhoTime software (Picoquant). WT.wdf and cad2_6_mutant.wdf = Original Raman mapping data .wdf files taken on a Renishaw InVia Raman microscope using WiRE 4 software. The data have been baseline subtracted. There are two files - one of a map of WT, the other is the lignin mutant. FLIM_Raman_replicates = ZIP archive of the SymPhoTime 64 workspace containing FLIM biological replicates labelled WT1, WT2 and WT3 for WT and cad_1, cad_2 for mutant. Also contains associated original Raman data (as .wdf files) taken on a Renishaw InVia Raman microscope using WiRE 4 software. The data have been baseline subtracted. Note WT1 represents a FLIM dataset that was taken without associated Raman mapping as a control experiment for FLIM with no prior Raman illumination.

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