Data for: Molecular characterization and expression analysis of the critical floral gene MdAGL24-like in red-fleshed apple

Published: 19 September 2018| Version 1 | DOI: 10.17632/kj29ndygdv.1
Xuesen Chen, Lin Xu, Zongying Zhang, Zhen Zhang, Su Mengyu, Hongcheng Fang, Shenghui Jiang, Haifeng Xu, Jing Zhang, Yicheng Wang, Nan Wang


We would like to submit the enclosed manuscript entitled " Molecular characterization and expression analysis of the critical floral gene MdAGL24-like in red-fleshed apple", which we wish to be considered for publication in Plant Science.. Compared with annual herbaceous plants, flowering is the premise of fruit bearing. The flowering of woody apple trees is relatively late because of its long juvenile phase that greatly affects the genetic improvement process of these plants. To better understand the molecular regulation of floral transition and flower development in red-fleshed apple, we isolated and characterized a floral MADS-box gene, MdAGL24-like, in this paper. MdAGL24-like shares sequence similarity with AGAMOUS-LIKE 24 (AGL24) from other species. It’s dynamically expressed in flowers, followed by roots and fruits. Like other transcript factors, MdAGL24-like was localized in the nucleus. MdAGL24-like could interact with MdSOC1 and MdAP1 in vivo and in vitro. MdAGL24-like and MdSOC1 could increase each other’s expression by binding the CArG motifs in their promoters. Unlike MdSOC1, MdAGL24-like couldn’t directly bind the downstream gene MdLFY. Ectopic overexpression of MdAGL24-like in wild-type Arabidopsis induced early flowering similar to the phenotypes induced by other AGL24 genes. Like AGL24 in Arabidopsis, MdAGL24-like could somewhat rescue the late-flowering phenotype of agl24. These results would clarify the molecular mechanism underlying flowering and provide a method of shortening the juvenile period in red-fleshed apples and other fruit trees. The work described has not been submitted elsewhere for publication, in whole or in part. All authors have seen the manuscript and approved to submit to your journal. Thank you very much for your attention and consideration.



Molecules, Agricultural Plant, Plant Development