Experimental Data-Effect of Fetal Bovine Serum in SH-SY5Y Proliferation

Published: 12-06-2020| Version 1 | DOI: 10.17632/kjm2fny4z4.1
Contributors:
Soha Ahmadi,
Qusai Hassan,
Kagan Kerman

Description

Fetal bovine serum (FBS) is the most common growth supplement used in the cell culture, which is a cocktail of low and high molecular weight biomolecules including growth-promoting and growth-inhibiting factors for cell attachment, growth, and proliferation. The high level of undefined proteins in FBS may cause unwanted cell stimulation.[1] Therefore, serum-free media is usually used during experiments. Unfortunately, when studying the long-term effects of drugs or chemicals, this serum starvation may lead to cell death in the control samples. In this experiment, we study the effect of the FBS in the proliferation of SH-SY5Y neuroblastoma cells. SH-SY5Y is a human-derived cell line used as an in vitro model of neuronal function and differentiation particularly to study neurodegenerative diseases such as Alzheimer’s disease and Parkinson’s disease.[2] We monitored the proliferation of SH-SY5Y cells in Dulbecco's Modified Eagle Medium (DMEM) containing different proportions of FBS for 7 days using a hemocytometer. These results can be used to design cytotoxicity experiments by choosing a suitable proportion of FBS in the DMEM for the SH-SY5Y in vitro system. References: (1) van der Valk, J.; Bieback, K.; Buta, C.; Cochrane, B.; Dirks, W. G.; Fu, J.; Hickman, J. J.; Hohensee, C.; Kolar, R.; Liebsch, M.; et al. Fetal Bovine Serum (FBS): Past - Present - Future. ALTEX 2018, 35 (1), 99–118. https://doi.org/10.14573/altex.1705101. (2) Lopes, F. M.; Schröder, R.; Júnior, M. L. C. da F.; Zanotto-Filho, A.; Müller, C. B.; Pires, A. S.; Meurer, R. T.; Colpo, G. D.; Gelain, D. P.; Kapczinski, F.; et al. Comparison between Proliferative and Neuron-like SH-SY5Y Cells as an in Vitro Model for Parkinson Disease Studies. Brain Res. 2010, 1337, 85–94. https://doi.org/10.1016/j.brainres.2010.03.102.

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