Raw HPLC-MS/MS data for 'The partitioning of fatty acids between membrane and storage lipids controls ER membrane expansion.'

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Data are the raw HPLC-MS/MS data uploaded in support of the publication ' The partitioning of fatty acids between membrane and storage lipids controls ER membrane expansion.' and relate to Figures 3 and EV5. Full experimental details are provided through the linked publication.

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Lipidomic data were obtained on a hybrid quadrupole Orbitrap mass spectrometer (Q Exactive, ThermoScientific) linked to an ultra-high performance LC system (Ultimate 3000, ThermoScientific) equipped with a reverse phase ACQUITY UPLC HSS T3 Column (100Å, 1.8 µm, 2.1 mm X 100 mm, Waters Corporation), held at 40°C in the column oven. Separation was achieved at a flow rate of 200 µL/min using solvent A; (60:40 v/v); water (Hypergrade for LC-MS, LiChrosolv®, MerckKGaA): acetonitrile (MSsuprasolve®, Sigma Aldrich), 10 mM ammonium formate (99.995%, Sigma Aldrich). And Solvent B; (90:10) isopropanol (Optima™LC/MS Grade, Fisher Scientific): acetonitrile, 10 mM ammonium formate. Lipid files are converted from Thermofisher (.RAW) to mzXML files using MSConvert, Proteowizard, chromatographic features were identified using MZMine and lipid identification were made using Lipidex. The following internal standards were used; SPLASH Lipidomix, Avanti Polar Lipids,10 μl. Full experimental details including strain grow conditions are available through the linked publication.

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