Microbial metabolic diversity in soils fertigated with residual vinasses

Published: 29 December 2022| Version 1 | DOI: 10.17632/kmp62j6kpd.1
Mariela Torres, Maria Virginia Angelicola, Hipolito Pajot, Carlos Gabriel Nieto-Peñalver


Vinasse is produced in large quantities in the sugar-ethanol industry. We analyzed the utilization of vinasse as culture medium for inoculants of agricultural relevance, and then we evaluated the ecological impact of the residual vinasses. Trichoderma harzianum MT2, a native isolate obtained from tomato rhizosphere, was cultured in sugarcane vinasse in single and in sequential co-cultures with Pseudomonas capeferrum WCS358 and Rhizobium sp. N21.2. After growth, residual vinasses were utilized for fertigation of trays containing soils not fertigated before. Microbial metabolic diversity in fertigated soils was evaluated with Biolog EcoPlates on day 14. The Average Metabolic Response was determined in EcoPlates incubated for 72 h and evaluated through the Principal Component Analysis. Results show that fertigation with residual vinasses from single culture of T. harzianum MT2 and sequential co-culture with P. capeferrum WCS358 has a lower short-term impact on the microbial metabolic diversity, than residual vinasse from sequential co-culture with Rhizobium sp. N21.2.


Steps to reproduce

P. capeferrum WCS358 and Rhizobium sp. N21.2 were cultured for 48 h at 30 °C in 10% sugarcane vinasse. Then, T. harzianum MT2 was inoculated, vinasse concentration was increased up to 50% and incubation was continued for another 72 h at 25 °C. In parallel, single culture of T. harzianum MT2 was also cultured in vinasse 50%. Trays containing soil were fertigated following local recommendations in Tucumán, Argentina, by aspersion on days 0 and 7 applying 1 L m-2 (irrigation sheets=1 mm). Trays were incubated at 25 °C for 14 days. Water contents in trays were daily adjusted to the initial values with distilled water considering the loss of weight. Microorganisms were extracted from 5 g of soil from each treatment after shaking at 200 rpm at 25 °C for 45 min with 45 mL of 0.9 % NaCl solution. The coarse particles were allowed to decant for 30 min at room temperature and 150 μL of these suspensions were utilized to seed wells of Biolog EcoPlates. Microplates were incubated at 25 °C for 7 days and the absorbance was measured on a daily basis at 590 nm (A590). The average metabolic response (AMR) for each treatment was calculated as the mean difference between the A590 of wells containing a carbon source (A590CS) and the control well with water (A590W): AMR=Ʃ(A590CS-A590W)/95.


Consejo Nacional de Investigaciones Cientificas y Tecnicas, Planta Piloto de Procesos Industriales Microbiologicos, Universidad Nacional de Tucuman


Applied Microbiology, Waste, Green Economy, Industrial Waste, Circular Bioeconomy


Consejo Nacional de Investigaciones Científicas y Técnicas

PIP 2021-2436

Consejo Nacional de Investigaciones Científicas y Técnicas

PUE 22920160100012CO

Agencia Nacional de Promoción Científica y Tecnológica

PICT 2019 Nº 03336

Agencia Nacional de Promoción Científica y Tecnológica

PICT 2018 N°01765

Universidad Nacional de Tucumán