Metagenomic sequencing of gut microbiota at the genus level in ketamine-addicted Sprague-Dawley rats

Description

In this data, we hypothesize that ketamine addiction leads to significant changes in the composition and function of gut microbiota in rats, and these changes are associated with addictive behavior and related physiological indicators. By conducting metagenomic sequencing of gut microbiota in ketamine addicted rats, we obtained detailed microbiome data and revealed differences in microbial composition between different experimental groups. Important findings include significant changes in the abundance of certain key microbial genera in the gut of ketamine addicted rats. Data analysis suggests that ketamine addiction may affect brain function and addictive behavior by regulating gut microbiota, providing a new perspective for understanding the role of the gut brain axis in addiction mechanisms.

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On the 2nd and 13th days of the CPP experiment (before and after model establishment), fresh fecal samples were collected from SD rats and stored at -80°C. Subsequent metagenomic analysis was conducted by the Wekemo Tech Group Co., Ltd. Shenzhen China. The CTAB method was employed to extract genomic DNA from 28 samples, followed by assessment of DNA integrity, purity, and concentration, construction of a DNA library, and sequencing of the qualified library. Raw metagenomic data from 32 samples was generated using the Illumina NovaSeq platform and preprocessed using Kneaddata software. Sample sequence reads were aligned against the NCBI NT nucleic acid database and RefSeq whole-genome database using Kraken2 and a custom-built microbial nucleic acid database. Relative abundance was predicted using Bracken. The sequences underwent quality control and de-hosting using HUMAnN2 software before comparison against the UniRef90 protein database using DIAMOND to obtain corresponding annotation information and a relative abundance table.

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