Chronic consumption of orange juice modifies urinary excretion of flavanone gut- derived metabolites through gut microbiota modulation
Description
This study evaluates the effect of acute and chronic consumption of orange juice (OJ) on the urinary excretion of gut- derived flavanone metabolites and the gut microbiota. Health volunteers ingested 500 mL of OJ for 60 days in a single-arm human intervention study.
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Bacterial DNA from the stool samples was extracted using the QIAamp Power Fecal Pro DNA Kit (QIAGEN, Germany). The V4 region of the 16S rRNA gene was amplified using the primers and conditions described previously (Kozich et al., 2013). Negative and positive controls were included in the amplification step. Amplicons were pooled in the Illumina MiSeq Reagent Kit v2 (500 cycles) for paired-end sequencing at a final concentration of 8 pM. A library was pooled at a density of approximately 820 k clusters/mm2.
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Funding
Fundação de Amparo à Pesquisa do Estado de São Paulo
Grant 2013/07914- 8