Raw data_Figures and Raw data_Supplementary Figures
Description
Raw data_Figures: Fig 1B-C, E-G Fig 2E-H Fig 3E-G Fig 4C-E Fig 5C-D Fig 6C-G Fig 7F-I Raw data_Supplementary Figures: Fig S1A-D Fig S2C-E, J-L Fig S3A-L, Q-S Fig S4E-G, I Fig S5A-D, H-K Fig S6C-D, G-J Fig S7A-B, D-F Highlights: * Both Piezo1 and Piezo2 dramatically enhance the mechanical activation of TREK/TRAAK K2P channels, slow down activation/deactivation and remove inactivation as demonstrated by electrophysiological patch clamp functional recordings * Piezo1/2 cause a pre-stress of TREK/TRAAK channels attributable to a local depletion of membrane cholesterol (patch clamp) * A native regulation between endogenous Piezo1 and TREK-1/2 occurs in mouse gingival fibroblasts, acting in concert to delay gum wound healing (patch clamp and in vivo) * Piezo1 and TREK-1 are in close proximity (not necessarily directly interacting), within the same nanodomain in the plasma membrane (co-immunoprecipitation, proximity assay, co-localization by confocal microscopy)