Data for quantitative research of mechanical properties of agar media with concentration gradient, and Arabidopsis root growth in these media
Description
The paper presents the data created for the published article entitled “Resistance from agar medium impacts the helical growth of Arabidopsis primary roots”.The data contains the real-time output forces of 0.5‒1.2% agar media from Bluehill software, and the forces on the agar surfaces changing with the increase of displacement. Oscillatory rheological experiments were employed to verify the stiffness results of 0.5‒1.2% agar media. Helix diameter and length of roots grown in gradient agar media for Col-0 and DR5-GUS Arabidopsis are exhibited.
Files
Steps to reproduce
Agar medium preparation The plant growth medium consisted of 0.5× Murashige, Skoog basal salts with Gamborg's B5 vitamins (Sigma M-0404), 1.5% sucrose and 0.5‒1.2% agar, and then was adjusted to pH 5.8 with KOH. The agar media were cast into rectangles (length = 40 mm, width = 20 mm, height = 12 mm) for compression test. Homogeneous agar media with a series of concentration from 0.5% to 1.2% were poured into the petri dishes (Diameter = 9 cm, glass) with thickness of 2 mm. A puncher with 2 cm diameter was pushed vertically into the agar layer to make round agar for oscillatory test. Uniaxial compression test and oscillatory test Under the conditions of 23◦C and 50% humidity, axial compression tests were performed on Electro PULS E1000. Loading rate was set as 1 mm per min, the test was finished when cross-head displacement reached 1.5 mm. The rheological properties were measured using a rotational rheometer. Root length and helical root shape for Col-0 and DR5-GUS comparison Arabidopsis thaliana roots were growing in agar media with increasing concentration as indicated in reference. Images of roots (n = 30) were captured in every culture condition. Thirty roots from each agar media were tested for root length and diameter of helix. For clear visualization of the root growth pattern in agar media, 7-day-old roots were observed under an Olympus microscope. Measurements of microscopy pictures were conducted by Image J software.