Data for quantitative research of mechanical properties of agar media with concentration gradient, and Arabidopsis root growth in these media
Description
The mechanical properties of the plant culture medium affect plant growth and development significantly. The paper presents the data created for the published article entitled “Resistance from agar medium impacts the helical growth of Arabidopsis primary roots”. The data contains the real-time output forces of 0.5‒1.2% agar media from Bluehill software, and the forces on the agar surfaces changing with the increase of displacement. Oscillatory rheological experiments were employed to verify the stiffness results of 0.5‒1.2% agar media. Helix diameter and length of roots grown in gradient agar media for Col-0 and DR5-GUS Arabidopsis are exhibited. The data reported herein contains Loading Force-Displacement Curve for 0.5‒1.2% agar media from uniaxial compression test (Fig. 1) and the relationship between agar concentration and moduli (Complex modulus and Elastica modulus) (Table 1). Data of root length (Table 2) and diameter of root helix (Table 3) in each agar media were presented when Col-0 and DR5-GUS Arabidopsis grow in agar media on the 7th day. Among the range from 0.5% to 1.2% agar media, no significant difference was found in both dimensions of the helical root and root length between Ecotype Columbia (Col-0) and DR5-GUS seeds (t-test, P >0.05) (Fig. 2). The raw data associated with Fig. 2 were exhibited in Table 2 and Table 3. Fig. 1 Loading Force-Displacement Curve for 0.5%-1.2% agar media from uniaxial compression test. Rectangle samples (length = 40 mm, width = 20 mm, height = 12 mm), area = 800 mm2. Common standard tests were used to determine the deformational displacement and Young’s modulus. The experiments were repeated more than three times for each agar concentration and similar results to those shown were observed. Fig.2 Average length and average helix diameter of 7-day-old primary roots of Col-0 and DR5-GUS in agar media. a. Col-0 and DR5-GUS comparison in 0.5%-1.2% agar media. b. Col-0 and DR5-GUS comparison in 0.5%-0.9% agar media. Col-0 and DR5-GUS comparison exhibited no significant difference (t-test, P>0.05). Table 1. The relationship between agar concentration and moduli (Complex modulus and Elastica modulus). Oscillatory rheological experiments for the effect of agar on the medium rheology were at constant frequency (0.1 Hz) and strain (0.2%). Table 2. Testing dataset with two genetypes of Arabidopsis roots. Length of 7-day-old primary roots of Col-0 and DR5-GUS in agar media with concentration of 0.5%-1.2%. This dataset is connected with Fig. 2. a. Col-0 and DR5-GUS comparison in 0.5%-1.2% agar media. Table 3. Testing dataset with two genetypes of Arabidopsis roots with helical growth pattern. Helix diameter of 7-day-old primary roots of Col-0 and DR5-GUS in agar media with concentration of 0.5%-0.9%.
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Steps to reproduce
Agar medium preparation The plant growth medium consisted of 0.5× Murashige, Skoog basal salts with Gamborg's B5 vitamins (Sigma M-0404), 1.5% sucrose and 0.5‒1.2% agar, and then was adjusted to pH 5.8 with KOH. The agar media were cast into rectangles (length = 40 mm, width = 20 mm, height = 12 mm) for compression test. Homogeneous agar media with a series of concentration from 0.5% to 1.2% were poured into the petri dishes (Diameter = 9 cm, glass) with thickness of 2 mm. A puncher with 2 cm diameter was pushed vertically into the agar layer to make round agar for oscillatory test. Uniaxial compression test and oscillatory test Under the conditions of 23◦C and 50% humidity, axial compression tests were performed on Electro PULS E1000. Loading rate was set as 1 mm per min, the test was finished when cross-head displacement reached 1.5 mm. The rheological properties were measured using a rotational rheometer. Root length and helical root shape for Col-0 and DR5-GUS comparison Arabidopsis thaliana roots were growing in agar media with increasing concentration as indicated in reference. Images of roots (n = 30) were captured in every culture condition. Thirty roots from each agar media were tested for root length and diameter of helix. For clear visualization of the root growth pattern in agar media, 7-day-old roots were observed under an Olympus microscope. Measurements of microscopy pictures were conducted by Image J software.