Data for: Activation of Macrophages by CpG DNA and LPS: an FTIR Spectroscopic Study

Published: 10 March 2020| Version 1 | DOI: 10.17632/kwnmk3fj54.1
Emrulla Spahiu, Senol Dogan, Jörg Schnauß, Mayda Gursel, Feride Severcan


Murine macrophage-like RAW 264.7 cells studied with ATR-FTIR. Control group was treated with phosphate-buffered saline (0.9% PBS; Sigma, USA), CpG DNA stimulated group was treated with 1 µM K3 ODN (Endotoxin-free; Integrated DNA Technologies, Belgium), and LPS stimulated group was treated with 0.5 µg/ml LPS (Sigma, USA). Four different times points following treatment were used for measurements to evaluate the kinetics of induced effects: 10 min, 30 min, 2 h, and 6 h.



Immunology, Spectroscopy, Biophysics, Fourier Transform Infrared Spectroscopy, Attenuated Total Reflectance-Fourier Transform Infrared Spectroscopy, Biophoton