The data of study about the chitosan-selenium nanoparticles on depression-like behaviour induced by fluoride in mice via JAK2-STAT3 pathway

Published: 6 August 2021| Version 1 | DOI: 10.17632/kxcgpn9ckv.1
Contributor:
Jinming Wang

Description

Data 18 firstly showed an unprecedented and very important finding was that 150 mg/L Sodium fluoride (NaF) resulted in the insufficient Se content in cortex. However, CS-SeNPs supplementation was beneficial to cortical Se retention in a dose-dependent manner and could antagonize fluoride-induced cortical Se depletion in data 18, which implied that oral administration of CS-SeNPs could be an effective way to supply Se. Further data 20 and 21 suggested that NaF decreased DA and NE content in the cortex, and 1 mg/kg·bw CS-SeNPs reduced the content of DA and NE, and 2 mg/kg·bw CS-SeNPs reduced the DA levels, while 0.5 mg/kg·bw CS-SeNPs had no obvious impact on the content of DA and NE, suggesting that long-term intake of 1, and 2 mg/kg·bw CS-SeNPs had potential negative effects on the monoamine nervous system. Interestingly, data 20 and 21 demonstrated that 0.5 and 1 mg/kg·bw CS-SeNPs protected against dopaminergic and noradrenergic damage caused by NaF in the cortex of mice in this research, which provided the theoretical basis for CS-SeNPs to exert antidepressant - like behavior. Raw data 22-24 showed that the decreased CORT secretion was induced by fluoride in the cortex, however, the expression of GR was significantly increased in the cortex exposed to fluoride, and there was no obvious change of CRF. 0.5 mg/kg·bw and 2 mg/kg·bw CS-SeNPs significantly increased the secretion of CORT and CRF, and 0.5 mg/kg·bw CS-SeNPs significantly decreased the expression of GR, suggesting that CS-SeNPs may improve the response of HPA axis to stress. What’s more, CS-SeNPs of 0.5 mg/kg·bw, 1 mg/kg·bw and 2 mg/kg·bw reestablished the fluoride-reduced CORT secretion. These results demonstrated that the mechanisms of fluoride-induced depression-like behavior were mainly related to the abnormal secretion of monoamine neurotransmitters, rather than the abnormal hyperactivity of the HPA axis. And 0.5 mg/kg·bw and 1 mg/kg·bw CS-SeNPs reversed the abnormal secretion of monoamine neurotransmitters. These data can be used for reference or comparison to further understand the neurotoxicity of long-term use of CS-SeNPs. At the same time, these data provide a comparison of the neurotoxicity of different doses of CS-SeNPs. 0.5 mg/kg·bw provides a relatively safe threshold for long-term consumption CS-SeNPs.

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The data were be obtained as follows: Raw data 16:The feeding period was 18 weeks, and the weight of the mice was recorded every week, and the changes in mental state and morphology were observed. The raw data were analysed by the GraphPad Prism 6.01 software. Raw data 17:Each mouse was weighed for its cortical weight and body weight, Cortex Visera Coefficient= cortical weight/ body weight×100%. The raw data were analysed by the GraphPad Prism 6.01 software. Raw data 18:Accurately weigh 0.1 g of the cortex and place it in a clean and dry microwave digestion tube, and do not touch the tube wall. Add 8 mL tissue digestion solution (A mixture of nitric acid and 30% hydrogen peroxide with a ratio of 9:1) and perform cold digestion overnight. On the second day, the digestion tube was placed in the microwave digestion tank (MARS6, CEM, China) for thermal digestion. Thermal digestion is carried out in the following stages: (1) In the first step, the power is set at 1600 W, the temperature is heated to 120 °C, the retention time is 5 min, and the climb time is 12 min; (2) In the second step, the power is set at 1600 W, the temperature is raised to 150 °C, the retention time is 5 min, and the climb time is 5 min; (3) The power of the third part is set at 1600 W, the temperature is raised to 180-190 °C, the retention time is 30 min, and the climb time is 5 min. After the digestion is over, keep it cool naturally and put it in the acid-driven processor (EHD-24, Beijing Annan Science& Technology Co., Ltd) with the condition at 120 ℃ for 90 min to drive away the acid. During this period, white smoke continued to emerge, and finally, a transparent tissue digestion solution was obtained. After cooling, dilute the transparent tissue digestion solution with ultrapure water into a 10 mL volumetric flask and filter with a 0.22 μm sterile syringe filter (PES, Tianjin Branch billion Lung Experimental Equipment Co., Ltd). The Se content of filtered digestion solution is determined by ICP-MS (iCAP QC, Thermo, USA) according to the measured results: Cortical Se content (ng/g)=[Ameasure (PPB) × 10 mL]/ weighing mass (g). The raw data were analysed by the GraphPad Prism 6.01 software. Raw data 19-24:100 mg cortical tissue was homogenized with 900 μL PBS solution in an ice bath in a homogenizer, then transferred to 1.5 mL EP tube, centrifuged at 3000 rpm at 4 ℃ for 15 min, and the supernatant was extracted. After the protein concentration was measured with the BCA kit (Beijing Solarbio Science & Technology Co., Ltd.), the standard operation was carried out according to the steps of the instructions according to the ELISA kit of the mouse-related indicators (Jiangsu Meimian industrial Co., Ltd). Finally, the concentration of homogenate calculated according to the standard curve is divided by the protein concentration of the sample, which is the content of protein per mg.