qRT-PCR Primers and Supplementary Images for Melasma Study
Description
This dataset contains supplementary materials for the study "Fractional Microneedle Radiofrequency Inhibits Melanin Metabolism, Microenvironmental Inflammation, and Senescent Fibroblasts in Melasma." The dataset includes two files: 1. Excel file (Supplemental_online_material.xlsx): Contains forward and reverse primer sequences for 16 genes analyzed by quantitative real-time PCR (qRT-PCR). Melanogenesis-related genes: - Tyr (Tyrosinase) - Mitf (Microphthalmia-associated transcription factor) - Trp-1 (Tyrosinase-related protein 1) Pro-melanogenic factors: - Hgf (Hepatocyte growth factor) - Scf (Stem cell factor) - Gdf-15 (Growth differentiation factor 15) Inflammation-related genes: - Tlr-2 (Toll-like receptor 2) - Tlr-4 (Toll-like receptor 4) - Il-17 (Interleukin 17) - Cox-2 (Cyclooxygenase 2) Angiogenesis-related gene: - Vegf (Vascular endothelial growth factor) Photoaging-related genes: - Mmp-1 (Matrix metallopeptidase 1) - Mmp-2 (Matrix metallopeptidase 2) - Mmp-3 (Matrix metallopeptidase 3) - Mmp-9 (Matrix metallopeptidase 9) - Collagen I All primers were designed and synthesized by Shanghai Sangon Biotech Co., Ltd. for use with guinea pig (Cavia porcellus) tissue samples. These sequences correspond to the methods described in Section 2.6 (Quantitative Real-Time PCR) of the manuscript. 2. Word document (Supplemental_online_material.docx): Contains supplementary images for Figure 1, including 2 high-resolution photographs (1024×1280 pixels, JPEG format) showing the experimental setup and treatment areas on guinea pig dorsal skin. File formats: - Microsoft Excel (.xlsx) - Primer sequences - Microsoft Word (.docx) - Supplementary images for Figure 1 Species: Guinea pig (Cavia porcellus) Total number of primer pairs: 16 Total number of supplementary images: 2
Files
Steps to reproduce
Total RNA was extracted from 30 mg of skin tissue samples in 1 mL of TRIzol reagent (Takara, Shiga, Japan) and homogenized using a high-speed, low-temperature tissue homogenizer (Servicebio, China). RNA (2,000 ng) was reverse-transcribed into cDNA using the PrimeScript RT reagent kit (Takara, Shiga, Japan), followed by the qRT-PCR performed on a BIO-RAD CFX system (Bio-Rad, Munich, Germany) using TB Green Premix Ex Taq II (Takara, Shiga, Japan). Five replicates per group were prepared. The relative mRNA levels were calculated using the 2–ΔΔCt method, with β-actin (ACTB) serving as the internal control. All primers were designed and synthesized by Shanghai Sangon Biotech Co., Ltd. The sequences of the primers used were listed in the Supplementary Material.