Single Cell RNAseq BAM files and processed data file for "Low intracellular calcium is an essential feature of hematopoietic stem cells"

Published: 13 Jun 2018 | Version 1 | DOI: 10.17632/kymy663rjv.1
Contributor(s):

Description of this data

The two .bam files uploaded contain aligned sequence data for sc-RNAseq of phenotypic HSCs sorted from culture after 14 days in high and low calcium chloride media.

The excel file contains the differential expression analysis of genes extracted from the three gene clusters (Cluster A, B, C) identified by multidimensional data visualization and analysis.

Experiment data files

Steps to reproduce

Cells were sorted on an BD Influx cell sorter and cells were sorted directly into lysis buffer. RNA extraction, cDNA library construction and sequencing and differential gene expression analysis was performed by the Columbia University Genome Center Sequencing Core. Data were visualized and statistical analyses were performed using the Seurat package in R Project.

Latest version

  • Version 1

    2018-06-13

    Published: 2018-06-13

    DOI: 10.17632/kymy663rjv.1

    Cite this dataset

    Snoeck, Hans (2018), “Single Cell RNAseq BAM files and processed data file for "Low intracellular calcium is an essential feature of hematopoietic stem cells"”, Mendeley Data, v1 http://dx.doi.org/10.17632/kymy663rjv.1

Institutions

Columbia University

Categories

Health Sciences, Stem Cell, Tissue Culture, Calcium, Hematopoietic Stem Cell

Licence

CC BY 4.0 Learn more

The files associated with this dataset are licensed under a Creative Commons Attribution 4.0 International licence.

What does this mean?

This dataset is licensed under a Creative Commons Attribution 4.0 International licence. What does this mean? You can share, copy and modify this dataset so long as you give appropriate credit, provide a link to the CC BY license, and indicate if changes were made, but you may not do so in a way that suggests the rights holder has endorsed you or your use of the dataset. Note that further permission may be required for any content within the dataset that is identified as belonging to a third party.