In-house database specific to bacteria from horses for MALDI Biotyper
Description
The dataset contains the main spectral profiles (MSPs) for MALDI Biotyper CA 3.2 System (Bruker Japan, Kanagawa, Japan) constitutes in-house database specific to bacteria from horses for identification with matrix-assisted laser desorption ionization-time of flight mass spectrometry (MALDI-TOF MS). MALDI-TOF MS is used for identification of bacterial species isolated from horses. however, some bacterial species isolated from horses are difficult to identify with MALDI-TOF MS because of insufficiencies in the reference database, and enriching the databases is expected to enhance the accuracy of MALDI-TOF MS identification. Here we created an in-house database including 271 bacterial isolates from horses. Bacterial isolates were subjected to ethanol / formic acid treatment, and spectra were gained using flexControl 3.4 software (Bruker Japan). The spectra were checked with flexAnalysis 3.4 software (Bruker Japan) to delete spectra differing from the cohort spectra and imported for generating MSPs using MBT Compass Explorer 4.1.7.0. software (Bruker Japan). MSPs were exported in a *.btmsp format for use with MALDI Biotyper systems.
Files
Steps to reproduce
To create main spectral profiles (MSPs), 271 bacterial isolates from horses shown in "species and strain.xlsx" file were subjected to ethanol / formic acid treatment. A few colonies were dissolved in 1.2 ml of 75% ethanol, centrifuged for 2 minutes at 13,000 rpm, and the supernatant removed. The pellet was dissolved in 20 µl of 70% formic acid and acetonitrile, centrifuged for 2 min at 13000 rpm. The supernatant was spotted (eight spots) onto a target plate and overlaid with α-cyano-4 hydroxycinnamic acid matrix solution. Each spot was measured three times (i.e., 24 spectra were gained for each isolate) using flexControl 3.4 software (Bruker Japan), and mass spectrometry spectra were acquired in the range of 2,000 to 20,000 m/z. The spectra were checked with flexAnalysis 3.4 software (Bruker Japan) and delete spectra differing from the cohort spectra. The remaining spectra were imported for generating MSPs using MBT Compass Explorer 4.1.7.0. software (Bruker Japan) and constructed an in-house database for bacteria from horses. These MSPs were exported as "in-house database.btmsp" file and this file can be imported with MBT Compass Explorer as a reference database.