Lipidome alterations in human prefrontal cortex during development, aging, and cognitive disorders. Yu et al.

Published: 18 January 2019| Version 1 | DOI: 10.17632/m4dt3z68s5.1
Qianhui Yu,
Zhisong He,
Dmitry Zubkov,
Shuyun Huang,
Ilia Kurochkin,
Xiaode Yang,
Tobias Halene,
Lothar Willmitzer,
Patrick Giavalisco,
Schahram Akbarian,
Philipp Khaitovich


DS1 represents the untargeted LC-MS measurement of lipidome composition of prefrontal cortex gray matter in 452 samples from 396 cognitively healthy individuals with ages spanning 100 years, as well as 16 autism, 26 schizophrenia and 5 Down syndrome patients. Measured using Orbitrap-XL, Thermo-Fisher, Germany, in both positive and negative ionization modes. Peak calling was done using Progenesis QI for data of positive and negative ionization modes separately. Peaks detected in at least 80% of the non-pooled samples and 90% of the pooled samples were retained. Log10-transformed peak intensities were normalized using quantile normalization. PFC transcriptomes of 72 selected individuals from the 396 ones measured in DS1 were measured by Illumina HiSeq 4000. The paired-ended reads were mapped to human genome hg38 using HiSat2, with reads overlapping with protein-coding genes annotated in GENCODE v24 using htseq-count and normalized using DESeq2. DS2 represents PFC lipidome measurement of 33 autism patients with their transcriptome measured in Liu et al. 2016. Peak calling was done using Progenesis QI with intensities normalized with upper-quartile normalization.



Icahn School of Medicine at Mount Sinai, Skolkovo Institute of Science and Technology, Shanghai Institutes for Biological Sciences Chinese Academy of Sciences


Human Aging, Lipidomics, Mental Disorder, Liquid Chromatography Mass Spectrometry, Prefrontal Cortex