Metagenomic dataset of Wedge-tailed shearwater (Ardenna pacifica)

Published: 6 April 2021| Version 2 | DOI: 10.17632/m4knvrw7jf.2
Contributor:
Subir Sarker

Description

A cutaneous tissue sample was collected from Wedge-tailed shearwater originating from Lord Howe Island, New South Wales (ID:15-1526, year of sampling: 2015, GPS location: 32.53° S, 159.08° E). The sample was collected with the permission of the Lord Howe Island Board (permit no. LHIB 02/14) under the approval of the University of Tasmania and Charles Sturt University Animal Ethics Committees (permit no. A0010874, A0011586, and 09/046). The genomic DNA was isolated according to our previously established protocol (Sarker et al., 2017) using the Qiagen Blood and Tissue mini kit (Qiagen, Germany). The extracted DNA has been stored at -20 °C at Charles Sturt University. The library preparation and sequencing was performed as previously described (Sarker et al., 2017) and cite SW1 paper. Sequencing data used in this study were analysed according to the previously established pipeline (Sarker et al., 2017; Sarker et al., 2019a; Sarker et al., 2019b) cite SW1 paper using Geneious (version 10.2.2, Biomatters, New Zealand) and CLC Genomics Workbench (version 9.5.4). Briefly, a total of 15.36 million reads with a read length of 150 bp was used to obtain the complete mitogenome of A. pacifica.

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Metagenomics, Seabird

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