Fig. 1. Comparison of colony growth and formation of clear zones in actinomycetes isolates through lipolytic test
The tributirin medium is widely used to test the lipase enzyme, which is exoenzyme, which is able to hydrolyze the medium containing tributyrin oil. Lipase plays a role in breaking the lipid complex into simple fragments. Tributyrin oil is a type of lipid group called triglycerides.