Gas chromatography/mass spectrum (GC/MS) Results for the Mineralization of Lissamine Green SF and Tetraethylrhodamine.
Description
The data consists of Common intermediates observed at 30minute intervals by Gas chromatography/mass spectrum (GC/MS) analysis after 180min Mineralization of Lissamine Green SF and Tetraethylrhodamine. Fragment compounds such as Phenol, 2,2'-methylenebis [6-(1,1-dimethylethyl)-4-methyl (m/z 340), 2,6-di-tert-butyl-4-(2,4,6-trimethyl benzyl) phenol (m/z 338), 3-Phenyl-2-ethoxypropylphthalimide (m/z 309), 13-Tetradecenyl acetate (m/z 256), Phenol, 2, 4-bis(1,1-dimethylethyl)-5-methyl (m/z 220) and Fumaric acid-ethyl-2-methylallyl ester (m/z 198) was observed for Lissamine Green SF (749.893 g/mol). Tetraethylrhodamine (479.02 g/mol) had common fragment compounds such as 1,2-benzene dicarboxylic acid, bis(2-ethylhexyl) ester (m/z 390), Dodecanoic acid, 4-nitrophenyl ester (m/z 321), 15-Hydroxypentadecanoic acid (m/z 258), 6-Hydroxy-4,4,5,7-tetramethyl-2-chromanone (m/z 220) and 2,5-Dimethyl-1, 5-hexadiene-3, 4-diol (m/z 142).
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Before the pollutant intermediate determination analysis, 10 ml of degraded samples were loaded onto a cartridge. The cartridge was conditioned with 2 ml methanol followed by 1 ml deionized water before the loading of the samples. The loaded sample was washed with 2 ml deionized water and left for 3 min to dry using the manifold. The samples were eluted by gravity with 2 ml methanol twice followed by 2 ml acetone. Extracted samples were concentrated using a stream of nitrogen gas over a water bath set at 45 oC. The sample was reconstituted with ethylacetate to 30ul and put into vials for GC analysis. SHIMADZA GC-MS QP 2020 was used to identify intermediate compounds from the transformed dyes. A blank was run first using ethylacetate. For the GC, a DB-5ms column of 30 m × 0.25 mm, 0.25 μm film thickness was employed. An injection pot temperature of 250° C and splitless injection mode were employed with helium as the carrier gas. For the MS, the ion source temperature was 210o C. It was run on scan mode set from m/z 50.00 to m/z 1030.