Potential probiotic Pediococcus pentosaceus M41 modulates its proteome differentially for tolerances against heat, cold, acid and bile stresses

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we have evaluated the differential proteomic responses of a potential novel probiotic Pediococcus pentosaceus M41 under heat, cold, acid, and bile stress conditions. We identified stress response proteins that could provide tolerances against these stresses and could be used as probiotic markers for evaluating stress tolerance. P. pentosaceus M41 was exposed to 50 oC for 2h (heat stress), 4 oC for 2h (cold stress), pH 3.0 for 2 h (acid stress), and 0.05% bile for 2h (bile stress). Proteomic analysis was carried out using 2D-IEF SDS PAGE and LC-MS/MS.

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