(supplementary data) Development of pH-sensitive Zerumbone-encapsulated Liposomes for Lung Fibrosis Via Inhalation Route

Description

Context and Purpose: This dataset provides supplementary material for the manuscript titled "Development of pH-sensitive Zerumbone-encapsulated Liposomes for Lung Fibrosis Via Inhalation Route." The data support the findings and analyses presented in the main manuscript, offering additional details on experimental procedures and results.

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Liposome Preparation 1. Add 7 mg of the liposomal composition of DPPC, Chol, OA, and ZER at 58:17:11:14 (%w/w) to a sealed glass tube. Then solubilize them in 1000 µL pure ethanol. 2. In the fume hood, dry the mixture under a gentle stream of nitrogen. 3. To the dried-down mixture, add 3 ml room temperature PBS solution and cover the end of the glass tube with the cap and vigorously shake it for 1 minute to completely resuspend the mixture. The result should be a milky, uniform suspension. 4. Fill the bath sonicator with distilled water. Place the sample in the middle of Elmasonic S 60H Ultrasonic device. The liquid level inside and outside of the tube should be the same and the temperature should be adjusted to 50 °C before placing the sample. Sonicate the suspension until it turns from milky to a little bit hazy. Check every 10 min; it will usually take approximately 30 min total sonication time. 5. Store the final product at 4 degrees C. The result is a suspension of small unilamellar vesicles (SUV).

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