Urinary Proteome Profiling Methodology Development Data

Published: 9 April 2018| Version 1 | DOI: 10.17632/mgsfsj7rv5.1
Ireshyn Govender


The data is linked to a manuscript which describes the development of a novel method for urinary proteome profiling. Various, commonly used, precipitation-based methods for urinary proteome sample preparation and analysis were compared to two novel methods. One being kit based and one being microsphere based. The entire workflow was completed for each method and analysed using LCMS. The uploaded data includes only the RAW LCMS data generated using a TripleTOF 6600 mass spectrometer. The data shows that the micro-sphere based method generates the most comprehensive proteome coverage whilst being the easiest and quickest to use. The kit based methods show greater robustness (due to being able to function efficiently under great temperature extremes) and proteome coverage is similar to that of the micro-sphere based approach. The kit-based method was the easiest and most feasible to implement in a clinical setting due to its robustness and ease of use. This data can be used to validate or compare against new or current methodologies applied in urinary proteomics research using mass spectrometry. Additionally the methodology presented in the research article can be applied to ones own research using urine as their biofluid of choice in clinical proteomics studies.



University of the Witwatersrand, Council for Scientific and Industrial Research


Proteomics, Proteomics Experimental Approach, Urinary Analysis