Sequences GB1-SEM1(68-107) and SEM1(68-107)

Published: 10 September 2021| Version 2 | DOI: 10.17632/mh36zhbv3y.2
Contributor:
Dmitriy Blokhin

Description

We attached nucleotide sequence of insertion GB1-SEM40 with his-tag (seq_gb1-sem40); amino acid sequences of GB1-SEM40 (file aa_seq_gb1-sem40) and SEM40 after TEV cleavage (file aa_seq_sem40_after_TEV); pETGB1::sem40 construction, annotated sequence (file petGB1-sem40); sequence of SEM40 amplificated from the Human genome, with restriction sites (NcoI, XhoI) and stabilizing TTTTTT tails (file sem40); fragment amplificated with T7-primers from pETGB1::sem40, It contains T7 promotor, GB1-SEM40 coding sequence, and T7 terminator (file T7sem40).

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Institutions

Kazanskij federal'nyj universitet

Categories

Natural Sciences

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