2-hr time-lapse imaging of A. thaliana leaf primordia

Published: 15 January 2024| Version 1 | DOI: 10.17632/mhd99xr6sz.1
Contributor:
Xin-Min Li

Description

Raw confocal stacks used for cell-cycle length measurement in the following study: TITLE: Cell-cycle-linked growth reprogramming encodes developmental time into leaf morphogenesis JOURNAL: Current Biology Article Type: Research Article Authors: Xin-Min Li, Hannah Jenke, Sören Strauss, Christos Bazakos, Gabriella Mosca, Rena Lymbouridou, Daniel Kierzkowski, Ulla Neumann, Purva Naik, Peter Huijser, Stefan Laurent, Richard S. Smith, Adam Runions, Miltos Tsiantis* *Corresponding Authors: Miltos Tsiantis (tsiantis@mpipz.mpg.de) Cellular growth of leaf primordia (genetic backgrounds and leaf nodes indicated) was monitored through live imaging at a high temporal resolution (2-hour time window). Plasma membranes (labeled with tdTomato fluorescence signals) were imaged using A Leica TCS SP8 Confocal Laser Scanning Microscope, and confocal images were channel-split and converted into .tif format stacks using FIJI. See the related publication for more details.

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Steps to reproduce

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Institutions

Max-Planck-Institut fur Pflanzenzuchtungsforschung

Categories

Image Database, Cell Cycle in Plant Cell, Biological Development, Leaf Studies

Funders

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