16S rDNA

Published: 29 June 2022| Version 1 | DOI: 10.17632/mjpr4v7dyd.1
菲妮 周


The present study was curcumin treatment of DSS-induced acute colitis mice and the study was divided into three groups (n = 8/group), control group (Con), model group, and treatment group. The data used mouse feces as a sample to detect the intestinal microbiota.


Steps to reproduce

1.Paired-end reads was assigned to samples based on their unique barcode and truncated by cutting off the primer sequence; Softwares:cutadapt Version:cutadapt-1.9 2.Paired-end reads into long amplicon fragments 16S(V3+V4): Softwares:FLASH Version:FLASH-1.2.8 ITS2: Softwares:PEAR Version:PEAR-0.9.6 3. denoise Softwares:qiime2 DADA2 Version:2019.7 4. sequence alignment Softwares:qiime2 feature-classifier Version:2019.7 5. Database for taxonomy SILVA(release 132) NT-16S(2019.04.05)


rDNA Analysis, Illumina MiSeq