In vitro production of antifungal phenolic acids by Hypomyces perniciosus, the causal agent of wet bubble disease of Agaricus bisporus
Wet bubble disease caused by Hypomyces perniciosus (syn: Mycogone perniciosa) is one of the major constraints in the production of button mushroom (Agaricus bisporus) worldwide. The crude culture filtrate and purified fraction of H. perniciosus induced leakage of cellular electrolytes from the mycelium of A. bisporus.
Steps to reproduce
Hypomyces perniciosus was grown in 500 ml conical flasks containing 250 ml of potato dextrose (PD) broth (Oxoid Ltd., UK) under stationary condition at 25°C for 3 weeks. The culture filtrate was collected by filtration through sterile filter paper and evaluated for its toxic activity towards Agaricus bisporus by using electrolyte leakage bioassay. A. bisporus was cultured in 500 ml conical flasks containing 250 ml of PD broth at 25°C for 2 weeks and mycelia were collected under aseptic conditions. Fifty mg of A. bisporus mycelium was transferred to a glass vial containing 10 ml of culture filtrate of H. perniciosus and the electrical conductivity of the solution was measured 3 h after incubation at 25°C using a conductivity meter (Jenway model 4510) and the data were recorded as μS cm-1 (Halo et al. 2018). The mycelial mat of A. bisporus treated with un-inoculated PD broth served as control.