Super and typical enhancer switching in early mammalian development involves eRNA mediated SWI/SNF recruitment

Published: 22 November 2023| Version 2 | DOI: 10.17632/mk8ttbt545.2
, Blaine Bartholomew,


Our data shows that the AT-hook domain of Brg1 is required for it's recruitment by an RNA dependent mechanism in distinct developmental stages. To further substantiate that the AT-hook Brg1 binds to the RNA, we purified the wild type and mutant AT-hook peptides and performed gel shift assay. Here, the gel images pertinent to the purification of the wild type and mutant AT-hook peptides and electrophoretic mobility shift assay (EMSA). The details of the experimental conditions for purification and EMSA are described in STAR Methods.



University of Texas MD Anderson Cancer Center


Protein, Electrophoretic Mobility-Shift Assay, Purification, Gel, Sodium Dodecyl Sulfate Polyacrylamide Gel Electrophoresis