Super and typical enhancer switching in early mammalian development involves eRNA mediated SWI/SNF recruitment
Published: 22 November 2023| Version 2 | DOI: 10.17632/mk8ttbt545.2
Contributors:
, Blaine Bartholomew, Description
Our data shows that the AT-hook domain of Brg1 is required for it's recruitment by an RNA dependent mechanism in distinct developmental stages. To further substantiate that the AT-hook Brg1 binds to the RNA, we purified the wild type and mutant AT-hook peptides and performed gel shift assay. Here, the gel images pertinent to the purification of the wild type and mutant AT-hook peptides and electrophoretic mobility shift assay (EMSA). The details of the experimental conditions for purification and EMSA are described in STAR Methods.
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Institutions
University of Texas MD Anderson Cancer Center
Categories
Protein, Electrophoretic Mobility-Shift Assay, Purification, Gel, Sodium Dodecyl Sulfate Polyacrylamide Gel Electrophoresis