A yeast FRET biosensor enlightens cAMP signalling dataset

Published: 17 June 2021| Version 1 | DOI: 10.17632/mknc4bn793.1
Contributors:
Dennis Botman, T. O'Toole,
, Frank Bruggeman, Johan van Heerden,

Description

The cAMP-PKA signalling cascade in budding yeast regulates adaptation to changing environments. We developed yEPAC, a FRET-based biosensor for cAMP measurements in yeast. We used this sensor with flow cytometry for high-throughput single cell-level quantification during dynamic changes in response to sudden nutrient transitions. We found that the characteristic cAMP peak differentiates between different carbon source transitions, and is rather homogenous among single-cells, especially for transitions to glucose. The peaks are mediated by a combination of extracellular sensing and intracellular metabolism. Moreover, the cAMP peak follows the Weber-Fechner law; its height scales with the relative, and not the absolute, change in glucose. Lastly, our results suggest that the cAMP peak height conveys information about prospective growth rates. In conclusion, our yEPAC-sensor makes possible new avenues for understanding yeast physiology, signalling and metabolic adaptation.

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Institutions

Vrije Universiteit Amsterdam

Categories

Carbohydrate Metabolism, Fluorescence Microscopy, Intracellular Signaling, Saccharomyces cerevisiae

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