Fluorophores and fluorescence microscopy

Published: 20-12-2016| Version 2 | DOI: 10.17632/mn8r329n6n.2
Edoardo Paluan


When utilizing a scanning confocal microscope, better resolution and image contrast is achieved when compared to traditional methods. Thin optical sections of living specimens, 100 micrometers thick can be imaged with such devices, which are capable of examining fluorescence emission between 400nm and 750nm. By using laser light of wavelength 470nm as an excitation source, a root cell sample stained with two different fluorescent dyes was examined. Two distinct peaks were notated at 643.98nm (red) and 537.72nm (green). The fluorophores used were deemed to be monolignol (green) and Peridinin Chlorophyll (red) as they matched the ratio of excitation/emission wavelength de-tected in the experiment.