Precise and Predictable CRISPR Chromosomal Rearrangements Reveal Principles of Cas9-mediated Nucleotide Insertion. Shou, li et al
Published: 27 June 2018| Version 1 | DOI: 10.17632/mrw942jfyr.1
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Jia ShouDescription
Original gel images for: Precise and Predictable CRISPR Chromosomal Rearrangements Reveal Principles of Cas9-mediated Nucleotide Insertion. Figure 4B, 4C and 4D : In vitro cleavage by Cas9 with biotin labeled DNA duplex and agarose-gel bands for Sanger sequencing. Figure S4A, S4B and S4C : In vitro cleavage by Cas9 and its nickases with biotin labeled DNA duplex and agarose-gel bands for Sanger sequencing. Figure S1C and S1J : Western blots verify depletion of Mre11 and CtIP. Figure S2C : Western blots verify depletion of FANCD2.
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Western Blot, Gel