articular cartilage development in tenascin-C deficient and wildtype mice

Published: 21 June 2018| Version 1 | DOI: 10.17632/mt3txxw53g.1
Contributors:
Martin Flück,
Bastian Gruber,
Michael Mienaltowski,
James MacLeod,
Johannes Schittny

Description

The data reports Mankin-scores, chondrocyte density and thickness of the tangential/transitional zone of the articular cartilage of tibial bone from wildtype and tenascin-C deficient mice at 1, 4 and 8 weeks of age.

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Breeding of Tenascin-C deficient and wildtype littermates of strain 129 SV Extraction of skeletal elements from the lower limb and fixation in 4% paraformaldehyde Preparation of tissue for paraffin embedding and sectioning Hematoxylin and eosin staining of tissue sections Microscopic assessment of cartilage structure based on the Mankin score Microscopic assessment of the thickness of the tangential/transitional zone of articular cartilage Morphometric assessment of cell density