Dataset 3 - Generated Peptide Libraries From Cnidaria Omics Data After Applying The In Silico Enzymatic Digestion Protocols

Published: 6 December 2024| Version 1 | DOI: 10.17632/mx4fkk4v8j.1
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Description

Single-enzyme proteolysis using five commonly employed enzymes in proteomics (AspN, Chymotrypsin, GluC, Proteinase K and Trypsin) to generate individual peptidomes from each protein database.

Files

Steps to reproduce

1- Single-enzyme proteolysis using 5 commonly employed enzymes in proteomics (AspN, Chymotrypsin, GluC, Proteinase K and Trypsin) with RapidPeptidesGenerator (RPG) v2.0.5 (https://rapid-peptide-generator.readthedocs.io/en/latest/) to the AMP precursor protein libraries generated in “Mendeley Data, V1, doi: 10.17632/myp4j56gpz.1" 2- Retention of peptides between 6 and 40 aminoacids in length 3 - Removal of duplicate peptides with Seqkit tool v2.6.1 (https://bioinf.shenwei.me/seqkit/download/) 4 - Eliminating peptides sharing above 98% of sequence identity with CD-HIT tool v4.8.1 (https://github.com/weizhongli/cdhit/releases) 5 - Exclusion of peptides containing non-standard aminoacids

Institutions

Universidade do Porto Centro Interdisciplinar de Investigacao Marinha e Ambiental, Universidad San Francisco de Quito Colegio de Ciencias de la Salud, Universidad Panamericana Aguascalientes Facultad de Ingenieria, Universidade do Porto Faculdade de Ciencias

Categories

Peptides, Biodiscovery, Omics, Antimicrobial

Licence