Dataset 3 - Generated Peptide Libraries From Cnidaria Omics Data After Applying The In Silico Enzymatic Digestion Protocols
Description
Single-enzyme proteolysis using five commonly employed enzymes in proteomics (AspN, Chymotrypsin, GluC, Proteinase K and Trypsin) to generate individual peptidomes from each protein database.
Files
Steps to reproduce
1- Single-enzyme proteolysis using 5 commonly employed enzymes in proteomics (AspN, Chymotrypsin, GluC, Proteinase K and Trypsin) with RapidPeptidesGenerator (RPG) v2.0.5 (https://rapid-peptide-generator.readthedocs.io/en/latest/) to the AMP precursor protein libraries generated in “Mendeley Data, V1, doi: 10.17632/myp4j56gpz.1" 2- Retention of peptides between 6 and 40 aminoacids in length 3 - Removal of duplicate peptides with Seqkit tool v2.6.1 (https://bioinf.shenwei.me/seqkit/download/) 4 - Eliminating peptides sharing above 98% of sequence identity with CD-HIT tool v4.8.1 (https://github.com/weizhongli/cdhit/releases) 5 - Exclusion of peptides containing non-standard aminoacids