Non-invasive 1H LF-NMR measurements of Wharton Jelly MSCs in vitro

Published: 8 April 2021| Version 1 | DOI: 10.17632/n3sfpcyp4z.1
Contributors:
,
,
Weronika Mazur,
,

Description

Dataset contains results from 1H low field nuclear magnetic resonance (LF-NMR) measurements of Wharton Jelly mesenchymal stem cells (MSCs) in vitro. A full quantitative characterization of MSCs' MR properties was possible by using different, complementary approaches. They encompassed various sample settings: MSCs cultured in a Petri dish, cell pellets in a cylindrical container and centrifuged cell suspensions in a glass pipette; experiments: 1D T1, 1D T2, 1D diffusion, 2D T1-T2 and D-T2; devices LF with a bore aperture and LF single-sided one with an extreme constant gradient strength. Complex NMR analysis was supported by random walk simulations. From the data, it was possible to determine MSCs' T1 and T2 relaxation times, cells and nuclei sizes, self-diffusion coefficients of the nucleus and cytoplasm, as well as to detect the influence of a single layer of cells on the effective diffusion coefficient of water and to identify apoptotic and necrotic cells death. Therefore, the dataset enables the obtainment of a full MR parameter set for MSCs and their biophysical characterization.

Files

Steps to reproduce

Raw_data_LF-NMR_bore_aperture requires the conduction of 1D or 2D Inverse Laplace Transform, while Raw_data_LF-NMR_single-sided (MoUSE) fitting of exponential functions.

Institutions

Uniwersytet Jagiellonski Collegium Medicum Wydzial Lekarski, Akademia Gorniczo-Hutnicza imienia Stanislawa Staszica w Krakowie

Categories

Physics, Biophysics, Mesenchymal Stem Cell, Cell Culture, In Vitro Relaxometry, Nuclear Magnetic Resonance Relaxation, Umbilical Cord-Derived Mesenchymal Stem Cell, Diffusion Imaging

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