The complete mitochondrial genome of the Common Rose butterfly, Pachliopta aristolochiae (Lepidoptera, Papilionoidea, Papilionidae) from Malaysia
Description
This data represents the complete mitochondrial genome of the Common Rose butterfly, Pachliopta aristolochiae, originating from Malaysia. It has a total of 15,235bp in length, consisting of 37 genes (13 PCGs, 22 transfer RNA, two ribosomal RNA) and an A+T rich control region. Genomic DNA was extracted using the Qiagen Blood and Tissue Kit (Qiagen, Valencia, CA) and fragmented using a Bioruptor® system. The library was prepared using NEBNext® Ultra™ II DNA Library Prep Kit for Illumina®. The sequence reads were generated using the Illumina NovaSeq 6000 platform with 150 paired-end mode (PE150). The assembly was carried out using NOVOPlasty v.4.2 and run through PALEOMIX BAM pipeline to assess the quality of the genome mapping. Then, the annotation was done using the MITOS v2 web server. Open Reading Frame (ORF) Finder was also used to verify predicted proteins and improve the genome annotation. The annotated mitogenome sequence file was converted into an accepted Genbank format using the GB2sequin web application. The circular mitogenome map was generated using OGDRAW. The mitogenome is available in Genbank under the accession number MZ781228.