Self-Organization of parS Centromeres by ParB CTP hydrolase

Published: 28 Oct 2019 | Version 2 | DOI: 10.17632/n5jjdrr55r.2
Contributor(s):
  • Young-Min Soh,
    Biochemistry, Genetics and Molecular Biology
    Chromosome Organization and Dynamics, Department of Fundamental Microbiology, UNIL, Switzerland
  • Iain Finley Davidson,
    Iain Finley Davidson
    Research Institute of Molecular Pathology (IMP), Vienna, Austria
  • Stefano Zamuner,
    Stefano Zamuner
    Laboratory of Statistical Biophysics, EPFL, Lausanne, Switzerland
  • Jerome Basquin,
    Jerome Basquin
    Crystallization Facility, Max Planck Institute of Biochemistry, Martinsried, Germany
  • Michael Taschner,
    Michael Taschner
    Chromosome Organization and Dynamics, Department of Fundamental Microbiology, UNIL, Switzerland
  • Florian Patrick Bock,
    Florian Patrick Bock
    Chromosome Orgranization and Dynamics, Department of Fundamental Microbiology, UNIL, Switzerland
  • Jan-Willem Veening,
    Jan-Willem Veening
    Systems and Synthetic Microbiology, Department of Fundamental Microbiology, UNIL, Switzerland
  • Paolo De Los Rios,
    Physics and Astronomy
    Laboratory of Statistical Biophysics, EPFL, Lausanne, Switzerland
  • Jan-Michael Peters,
    Jan-Michael Peters
    Research Institute of Molecular Pathology (IMP), Vienna, Austria
  • Stephan Gruber
    Biochemistry, Genetics and Molecular Biology
    Chromosome Organization and Dynamics, Department of Fundamental Microbiology, UNIL, Switzerland

Description of this data

Dracala spotting assay, ITC measurements, Malachite Green Assay, in vitro and in vivo crosslinking , DNA loading assay, Sequence alignment used for DCA, Single Molecule Imaging

Experiment data files

  • Figure1
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  • Figure2
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    • Malachite Green
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      Malachite Green Assay raw data from plate reader. Raw data from the standard were used to make a standard curve and extrapolate the standard equation. Standard equation was used to calculate free phosphate in the ParB malachite green assay. These values were multiplied by the dilution factor 4, and the result was then divided by 60 minutes to get the relative rates. Finally, the relative rates were divided by the protein concentration, 12 µM (Fig2A Experiment 1), 8 µM (Fig2A Experiment 2 and3, Fig2B experiment 1,2,and3), to get the absolute rates. *All standards are of the following phosphate concentrations: 40 µM, 32, 24, 16, 12, 8, 4, 0 µM

  • Figure3
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  • Figure4
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  • Supplementary Figures
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    • Fig S3A Malachite Green Assays
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      Raw data from the standard were used to make a standard curve and extrapolate the standard equation. Standard equation was used to calculate free phosphate in the ParB malachite green assay. These values were multiplied by the dilution factor 4, and the result was then divided by 60 minutes to get the relative rates. Finally, the relative rates were divided by the protein concentration, 8 µM, to get the absolute rates. *All standards are of the following phosphate concentrations: 40 µM, 32, 24, 16, 12, 8, 4, 0 µM

    • Fig S3B Malachite Green Assays
      Cite

      Raw data from the standard were used to make a standard curve and extrapolate the standard equation. Standard equation was used to calculate free phosphate in the ParB malachite green assay. These values were multiplied by the dilution factor 4, and the result was then divided by 60 minutes to get the relative rates. Finally, the relative rates were divided by the protein concentration, 8 µM, to get the absolute rates. *All standards are of the following phosphate concentrations: 40 µM, 32, 24, 16, 12, 8, 4, 0 µM

Related links

Latest version

  • Version 2

    2019-10-28

    Published: 2019-10-28

    DOI: 10.17632/n5jjdrr55r.2

    Cite this dataset

    Soh, Young-Min; Davidson, Iain Finley; Zamuner, Stefano; Basquin, Jerome; Taschner, Michael; Bock, Florian Patrick; Veening, Jan-Willem; De Los Rios, Paolo; Peters, Jan-Michael; Gruber, Stephan (2019), “Self-Organization of parS Centromeres by ParB CTP hydrolase”, Mendeley Data, v2 http://dx.doi.org/10.17632/n5jjdrr55r.2

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Institutions

Universite de Lausanne

Categories

Biochemical Assay

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