Prompting immunostimulatory activity of curdlan with grafting methoxypolyethylene glycol

Published: 9 June 2022| Version 1 | DOI: 10.17632/n66vmsb2kk.1
Contributor:
Lei Luo

Description

In this study, we used methoxypolyethylene glycol (mPEG) to modify CUR and unwind the triple helix structure to prompt the immunostimulatory activity. CUR-mPEG was synthesized through the substitution of hydroxides on CUR with mPEG-OH (Scheme 1). The physico-chemical properties of CUR-mPEG were characterized, including Fourier-transform infrared spectroscopy (FT-IR), hydrogen nuclear magnetic spectroscopy (1H-NMR), solubility, scanning electron microscopy (SEM), and Congo red tests. The release of tumor necrosis factor (TNF)-alpha and interleukin (IL)-6 from macrophages with the treatment of CUR-mPEG in vitro were assessed by enzyme-linked immunosorbent assay (ELISA). The cytotoxicity was characterized by CCK-8 and live/dead cell staining tests, and its antibacterial property was characterized by phagocytosis test.

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Institutions

Jinan University

Categories

Biomaterials, Carbohydrate

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