Data for: OsDSSR1, a novel small peptide, enhances drought tolerance in transgenic rice

Published: 13-03-2018| Version 2 | DOI: 10.17632/n6h5tmg2dr.2
Contributors:
Yanchun Cui,
Shufeng Song,
Can Peng,
Manling Wang,
Xuming Yin,
Mingjuan Li,
Xinjie Xia,
Guoyun Xu,
Chunyong Li

Description

RNA-seq analysis was carried out using transgenic lines OX37. Fourteen-day-old rice seedlings were treated with or without 20% solutions of PEG6000 for 6h. Total RNA was isolated with Trizol reagent (Invitrogen, USA) from seedlings of transgenic and 93-11 plants grown under normal or PEG conditions. Material from 10 plants of each genotype was pooled for RNA extraction. The concentration and quality of each RNA sample were determined using a NanoDrop 2000TM micro-volume spectrophotometer (Thermo Scientific, Waltham, MA, USA) and gel electrophoresis. The rice RNA samples were sent to BGI (The Beijing Genomics Institute at Shenzhen, China) for sequencing.

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