Dual X-ray Absorptiometry of Btg2 knockout mice during starvation
Btg2 wild type (Btg2-WT) and Btg2 knockout (Btg2-KO) mice of male and of female were fasted for 48 hours and then re-fed for 24 hours. On each time point, body weight of each mouse, lean body mass and fat mass were measured by dual-energy X-ray absorptiometry (DEXA). We found Btg2-KO mice consume less fat mass during starvation in both sexes, and have lower bone mineral density in female.
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The lean body mass, fat mass, and bone mineral density of twenty weeks old mice were measured by the non-invasive manner with the Peripheral Instantaneous X-ray Imager (PIXImus) (GE Lunar Corp, Madison, WI, USA). An anesthetized mouse (by Ketamin + Zoletil) was placed on the exposure panel with ventral side facing down and all extremities were stretched out and bent within the exposure panel as below. Trunk (from diaphragm to the start of tail) was selected to measure lean body mass, fat mass, and the bone mineral density (BMD) of each mouse was measured in femur. Detection threshold was lowered from 1325 to1305 to measure fat and lean mass. After measurement, the mice were placed on a warm pad until recovery to maintain body temperature. Student t-test was applied to compare statistical difference between Btg2-WT and Btg2-KO and outlier is not included in statistical analysis.