DPPH Radical Scavenging Activity of Rutin and Microalgal Extracts at Various Concentrations
Description
This study aimed to determine the antioxidant activities of various microalgae species isolated from Giresun rivers and to compare them with rutin. Our hypothesis was that the green microalgae species isolated from Giresun rivers would exhibit high antioxidant capacities and would show significant differences in antioxidant activity when compared to rutin. We anticipated that rutin would demonstrate higher DPPH radical scavenging activity compared to the tested microalgae species, indicating a stronger antioxidant capacity. Additionally, we expected to observe significant differences in antioxidant activities among the microalgae species. Statistical analyses revealed significant differences in DPPH inhibition percentages among the microalgae species (p < 0.001). This indicates that the antioxidant activities of different microalgae species show distinct differences. Concentration levels also had a statistically significant effect on DPPH inhibition (p < 0.001), demonstrating that varying concentrations impact antioxidant activity. Tukey HSD test results showed that rutin exhibited significantly higher DPPH radical scavenging activity compared to Chlorella vulgaris and Chlorococcum hypnosporum (p < 0.001). This finding suggests that rutin is more effective than these microalgae species. Significant differences were found between Chlorolilaea pamvotia and Chlorococcum hypnosporum (p < 0.01), indicating that their antioxidant activity levels are not similar. On the other hand, differences between Chlorella vulgaris and Desmodesmus opoliensis were not statistically significant (p > 0.05), suggesting that while variations in antioxidant activity exist, they may not be as pronounced as those observed between microalgae and rutin. The data were collected using the DPPH radical scavenging test, a common method for measuring antioxidant activity. This test assessed how effectively different microalgae species and rutin could neutralize DPPH radicals. Concentration levels were varied to evaluate their impact on antioxidant activity. The findings highlight that rutin exhibits superior antioxidant activity compared to other microalgae species and that there are notable differences in antioxidant activities among the microalgae species themselves. These results emphasize rutin's strong antioxidant properties and provide insights into the potential uses and effectiveness of different microalgae species in antioxidant research.