A comprehensive analysis of the faecal microbiome and metabolome of Strongyloides stercoralis infected volunteers from a non-endemic area

Published: 5 October 2018| Version 1 | DOI: 10.17632/n86dtjvmbv.1
Timothy Jenkins, Fabio Formenti, Cecilia Castro, Chiara Piubelli, Francesca Peradin, Dora Buonfrate, Domenico Otranto, Jules Griffin, Lutz Krause, Zeno Bisoffi, Cinzia Cantacessi


In this study, we explore the impact of sole, natural infections by the human parasite Strongyloides stercoralis on the faecal gut microbiota and metabolic profiles of a cohort of human volunteers from northern Italy (S+), pre- and post-anthelmintic treatment, and compare the findings with data obtained from a cohort of uninfected controls from the same geographical area (S-). The collected data was obtained through high-throughput sequencing of bacterial 16S rRNA using an Illumina Miseq and nuclear magnetic resonance (NMR) and gas chromatography - mass spectrometry analyses of raw faecal samples. The data set includes four types of files: Raw joined read files - 1 per sample (demultiplexed): e.g. S+pre-treatment1.fasta OTU table created using Qiime including all of the taxonomic information: OTU_table.biom A table containing the metabolite quantification from the NMR and GC-MS analyses. These tables have been normalised to total area: Metabolomic_data_NMR_GC-MS_nomalised_to_total_area.xlsx Metadata file containing the relevant contextual information for each sample: Supplementary table S1.xlsx


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Microbiome, Metabolomics, Gut, Helminth, Parasite, Metabolism, Helminth Infection, Gut Microbiota, Gut Microbiome, Strongyloides, Metabolome